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This study provides an integrated genome wide analysis of the BAHD family coupled with in vivo functional validation via gene silencing, revealing that all four NtHQT isoforms contribute to CGA biosynthesis and stress adaptation, despite their divergent enzymatic activities and expression patterns

De novo transcriptome assembly was accomplished using Trinity 57 with min_kmer_cov set to 2 by default and all other parameters in the default settings

To load a crude extract from 0.2 mg FW, a crude extract was 5-fold diluted with lysis buffer (0.04 mg/L) and, then, 5 L of sample solution was applied

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